aga model group  (MedChemExpress)

Journal: Bioactive Materials

Article Title: Sustainability of functional hair follicle activity: Impact of spatially anchored multifunctional tetrahedral framework nucleic acids

doi: 10.1016/j.bioactmat.2025.11.006

Figure Lengend Snippet: Establishment of AGA model and treatments. (A) Schematic diagram of the establishment of the AGA model and treatments. (B) Immunofluorescence staining, section scanning, and whole-mount imaging of clarified tissues demonstrating the transdermal efficacy of TQC and tFNAs. Blue: DAPI; Red: Cy5-TQC/Cy5-tFNAs. (C) Time-course observations of hair growth of samples at 1, 4, 7, 10, 14, 15, 18, 21, 24, and 28 days (n = 5). (D) Whole-mount imaging and magnified views of treated samples shot with a Light Microscopy: Control, AGA, Minoxidil, Que, tFNAs, and TQC (n = 5). (D) (E) Longitudinal-sectional and cross-sectional HE staining of treatment groups: Control, AGA, Minoxidil, tFNAs, Que, and TQC. Scale bars: 3 mm (overview), 300 μm/500 μm (magnified regions). Black triangle: Sebaceous gland; black arrowheads: Hair shaft; yellow arrowheads: Hair bulb; green arrowheads: Hair papilla; blue arrowheads: Melanocyte (n = 5). (F) Quantitative image analysis of whole mount hair follicles density (number/view) in figure D. Data are expressed as mean ± SD (n = 5). Statistical significance was determined by one-way ANOVA with Tukey's post hoc test. (G) Quantitative image analysis of hair follicle density (number/mm) in cross-sectional HE staining (figure E). Data are expressed as mean ± SD (n = 5). Statistical significance was determined by one-way ANOVA with Tukey's post hoc test. (H) Ratio of anagen to telogen follicles in longitudinal-sectional HE staining (figure E). Data represent mean ± SD (n = 5) and are analyzed using one-way ANOVA with Tukey's test. The following symbols denote differences compared to each group: (∗) control, (#) AGA, (&) AGA + Minoxidil, (@) AGA + tFNAs, ($) AGA + Que. Significance levels are: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Article Snippet: After 14 days’ model establishment, the mice were assigned to the following groups: AGA model group, Minoxidil ( U10858 , MCE, USA, 5 %)-treated group, Que (20 μM)-treated group, tFNAs (250 nM)-treated group, and TQC (tFNAs/Que: 250 nM/20 μM)-treated group.

Techniques: Immunofluorescence, Staining, Imaging, Light Microscopy, Control

Journal: Bioactive Materials

Article Title: Sustainability of functional hair follicle activity: Impact of spatially anchored multifunctional tetrahedral framework nucleic acids

doi: 10.1016/j.bioactmat.2025.11.006

Figure Lengend Snippet: The role of TQC in mediating Wnt/β-catenin signaling and hair follicle regeneration in AGA mice. (A) Western blot analysis of β-catenin, GSK3β, CK-1α, and APC protein expression in different treatment groups. GAPDH was used as an internal control (n = 5). (B) Quantitative analysis of protein expression levels relative to the control group. Data are expressed as mean ± SD (n = 5). Statistical significance was determined by one-way ANOVA with Tukey's post hoc test. (C) Immunohistochemical staining of GSK3β, CK-1α, and APC in skin tissue sections. Magnified images show representative areas of staining. Scale bars: 3 mm (original magnification) and 500 μm (magnified), (n = 5). (D) Immunofluorescent staining of Ki67 (green) and KRT14 (red) in skin sections. DAPI (blue) was used for nuclear staining. Merged images show the co-localization of Ki67 and KRT14. Scale bars: 2 mm (original magnification) and 200 μm (magnified). (E) Quantification of β-catenin, GSK3β, and CK-1α protein expression on immunofluorescent staining sections calculated by ImageJ (n = 5). Data are expressed as mean ± SD (n = 5). Statistical significance was determined by one-way ANOVA with Tukey's post hoc test. (F) Quantification of Ki67 and KRT14 positive area. Data are expressed as mean ± SD (n = 5). Statistical significance was determined by one-way ANOVA with Tukey's post hoc test. The following symbols denote significant differences compared to each group: (∗) control, (#) AGA, (&) AGA + Minoxidil, (@) AGA + tFNAs, ($) AGA + Que. Significance levels are: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Article Snippet: After 14 days’ model establishment, the mice were assigned to the following groups: AGA model group, Minoxidil ( U10858 , MCE, USA, 5 %)-treated group, Que (20 μM)-treated group, tFNAs (250 nM)-treated group, and TQC (tFNAs/Que: 250 nM/20 μM)-treated group.

Techniques: Western Blot, Expressing, Control, Immunohistochemical staining, Staining

Journal: Translational Psychiatry

Article Title: NADPH alleviates LPS-induced neuropathology and depression-like behaviors by suppressing microglial inflammatory response

doi: 10.1038/s41398-025-03761-1

Figure Lengend Snippet: A qRT-PCR analysis IL-1β, IFN-γ, TNF-α, Aif1, CX3CR1, TREM2 relative gene expression level in the hippocampus of mouse (n = 5 mice per group, F (3, 12) = 12.51, F (3, 12) = 9.885, F (3, 12) = 22.82, F (3, 16) = 17.69, F (3, 16) = 8.891, F (3, 16) = 5.921). B Effects of NADPH treatment and LPS on oxidative stress products (SOD, GSH-PX, MDA) and CORT content in the hippocampus of mouse (n = 6 mice per group, F (3, 20) = 68.93, F (3, 20) = 50.99, F (3, 20) = 21.85, F (3, 20) = 10.52). Data are represented as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.001 by one-way ANOVA followed by Tukey’s test. C Representative images of microglial Iba1 immunofluorescence staining and Imaris-reconstructed 3D models across experimental groups; Scale bar = 3 μm. D Semi-automated quantification of microglial activation. Representative bar graphs of Iba1 expression and 3D morphological measurements of microglia. Each symbol represents an individual microglial cell. Data were analyzed from 5 cells per group, derived from at least 5 mice (n = 5–6 per group, intensity mean, F (3, 20) = 11.69, soma volume, F (3, 16) = 10.52, dendrite length, F (3, 19) = 20.66, No. of dendrite branch Pts, F (3, 19) = 11.57, No. of segment, F (3, 19) = 13.31, No. of dendrite terminal Pts, F (3, 19) = 15.01). E Sholl analysis of microglia. (10–20μm, *, control vs LPS+NaCl, p <0.05. 18–22μm #, LPS+NaCl vs LPS + NADPH, p <0.05). Data are represented as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.001 by one-way ANOVA followed by Tukey’s test.

Article Snippet: C Representative images of microglial Iba1 immunofluorescence staining and Imaris-reconstructed 3D models across experimental groups; Scale bar = 3 μm.

Techniques: Quantitative RT-PCR, Gene Expression, Immunofluorescence, Staining, Activation Assay, Expressing, Derivative Assay, Control